The use of ORF2 and ORF3 proteins for creating an enzyme-linked immunosorbent assay for viral hepatitis E

Due to the lack of national analogies a prototype enzyme-linked immunosorbent assay (ELISA) test system was developed for qualitative determination antibodies class G hepatitis E virus (HEV) based on using antigenic polypeptides ORF2 and ORF3 3rd genotype (FSBSI «I.I. Mechnikov Research Institute Vaccines Serums», Russia). The results experimental ELISA conducted in parallel with reference «DS-ELISA-ANTI-HEV-G» (NPO Diagnostic Systems, Russia) are presented. working concentrations antigens were 2.5 µg/mL 0.25 µg/mL, respectively. They tested 200 donors patients’ serum samples. article reflects sensitivity, specificity reproducibility results. demonstrated high efficiency detection specific anti-HEV-IgG.